Math Problem Statement
Suppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b=1.00 cm).
The absorbance of the solution at 427 nm is 0.30 . If the molar absorptivity of yellow dye at 427 nm is 27400 M–1cm–1, what is the concentration of the solution in M?
Solution
To calculate the concentration of the solution using the given data, we can use Beer-Lambert's Law, which is:
Where:
- is the absorbance (0.30),
- is the molar absorptivity (27,400 Mcm),
- is the path length (1.00 cm),
- is the concentration in molarity (M).
We can rearrange this formula to solve for the concentration :
Now we plug in the values:
Let me calculate that for you.The concentration of the yellow dye solution is approximately .
Would you like more details or have any questions about the calculation? Here are five related questions:
- What happens to the concentration if the absorbance increases?
- How does the molar absorptivity affect the sensitivity of absorbance measurements?
- Can Beer-Lambert’s law be applied for highly concentrated solutions?
- What are the limitations of the Beer-Lambert law?
- How would you measure the absorbance of a different wavelength for the same dye?
Tip: For very dilute solutions, absorbance values might be too small to measure accurately, so ensuring a sufficiently concentrated sample is key.
Ask a new question for Free
By Image
Drop file here or Click Here to upload
Math Problem Analysis
Mathematical Concepts
Chemistry
Beer-Lambert Law
Algebra
Formulas
A = ε × b × c
c = A / (ε × b)
Theorems
Beer-Lambert Law
Suitable Grade Level
Undergraduate (General Chemistry)
Related Recommendation
Calculate the Extinction Coefficient (ε) Using Beer-Lambert Law
Calculate Concentration of Dye Using Beer-Lambert Law (Absorbance 0.25 at 542 nm)
Calculating Concentration and Molar Absorptivity Using Beer-Lambert Law and Dilution
Calculate Concentration Using Beer-Lambert Law with Absorbance 0.60
Calculate Molar Concentration Using Calibration Curve with Absorbance of 0.212