The resolution ($R_s$) between two chromatographic peaks can be calculated using the formula:

$R_s = \frac{2(t_{2} - t_{1})}{w_{b1} + w_{b2}}$

Where:

• $t_1$ and $t_2$ are the retention times of the two compounds,
• $w_{b1}$ and $w_{b2}$ are the base widths of the two peaks.

Step 1: Assign the values

• $t_2 = 12.5 \, \text{min}$, $t_1 = 6.2 \, \text{min}$,
• $w_{b1} = 5.0 \, \text{min}$, $w_{b2} = 5.5 \, \text{min}$.

Step 2: Plug the values into the formula

$R_s = \frac{2(12.5 - 6.2)}{5.0 + 5.5}$

$R_s = \frac{2(6.3)}{10.5}$

$R_s = \frac{12.6}{10.5} \approx 1.2$

Step 3: Determine if the resolution is above baseline

• A resolution of $R_s \geq 1.5$ is generally required for complete baseline separation.
• Here, $R_s \approx 1.2$, which is not sufficient for baseline separation.

---

Final Answers:

Blank 1 (Numeric Answer): 1.2
Blank 2 (Word Answer): no

---

Do you have further questions, or would you like clarification on how the resolution is calculated?

---

Related Questions:

1. How does the resolution affect the accuracy of compound identification in chromatography?
2. What are other factors (besides peak widths) that influence chromatographic resolution?
3. How can chromatographic conditions be adjusted to improve resolution?
4. What is the significance of baseline separation in quantifying compounds?
5. Can increasing the column length improve resolution? Why or why not?

Tip:

To improve resolution, you can experiment with changing the mobile phase composition, temperature, or flow rate to optimize the separation.